Figure 1.

Alignment of amino acid sequences of A. aculeatus (asp; accession no. AAA67426), T. reeseii (trr; accession no. AAA34208), Arabidopsis (AtMAN1, expressed sequence tag accession no. AAD20927; and AtMAN2, translated protein sequence from genomic clone K1F13.9 [www.kazusa.or.jp/kaos/]), and tomato post-germinative (LeMAN1, accession no. AAB87859) and germinative (LeMAN2, cDNA accession no. AF184238) mannanases. The putative signal peptide (22 amino acids) in LeMAN2 is underlined. The double-underlined amino acids indicate a possible N-glycosylation site, and putative catalytic sites conserved across fungal and plant mannanases are indicated by asterisks (Bewley et al., 1997). Amino acids identical in three or more of the sequences are highlighted by reverse shading.