Figure 6.

Tissue printing and hybridization of germinating (24-h-imbibed) and germinated (5-mm radicle length) tomato seeds. Seeds were bisected, pressed onto a membrane, and hybridized with antisense riboprobes for LeMAN2 (A and B), LeMAN1 (C and D), and G46 (constitutively expressed ribosomal protein mRNA used as a printing control; E and F). The dark areas indicate hybridization. The arrows in D indicate the remaining endosperm cap tissue in the germinated seeds, which does not hybridize with the LeMAN1 riboprobe. Representative images are shown of more than 20 individual seeds printed and hybridized for each condition.