Figure 8.

Northern blot of total RNA and gel diffusion assay for mannanase activity of protein extracts from the endosperm caps of wild-type tomato seeds imbibed for 24 h in water or 100 μm ABA, and gib-1 mutant seeds imbibed for 24 h in water, 100 μm GA, or 100 μm GA plus 100 μm ABA. The northern blot was hybridized with a LeMAN2 riboprobe. Endo-β-mannanase activity was 31.6 pmol min⁻¹ in all cases except gib-1 seeds incubated in water, where activity was 0 pmol min⁻¹.