Figure 4.

Hematopoietic Reconstitution after HSCGT

The following analyses were performed 9–11 months after transplantation of Lin⁻ cells transduced at MOI 10. (A) FACS analysis of peripheral blood, bone marrow, and spleen cells. Cells were stained with antibodies against CD45.2 and CD3, CD19, and CD11b for detection of, respectively, T, B, and myeloid leukocytes (n = 3–12 mice/group). The horizontal line represents the median; *p < 0.05, **p < 0.01. (B) Colony-forming unit assay. BM cells were cultured and granulocyte-monocyte progenitors (CFU-GM) were counted, n = 1–6 mice /group. The horizontal line represents the median.