Table 1. Axon terminal distribution and firing patterns of identified orchid cells.
All projection axons traveled in the dorsal fornix. *Varicosities were sampled from different numbers of brain sections per labeled neuron. **Main axon observed dorsal of SUBd/PrSd, too weak to observe collaterals. ***Main axon faded away just rostral of caudo-dorsal EC. Abbreviations: c, fine axon collaterals observed; u, unknown/unavailable; n.o., not observed. Spike burst defined as >3 spikes with interspike intervals (ISIs) < 40 ms. Firing rates and burst incidence (Hz) are expressed as mean of 1 s bins ± s.d.; intraburst frequency (Hz) as mean ±s.d.; burst duration and interburst interval (ms) are expressed as median and interquartile range. Abbreviations: LM, locomotion; SM, small movements including limbs, tail, and shifts in posture; WS, high-frequency whisking and/or sniffing in the absence of other movements; IM, immobility. KS, Kolmogorov-Smirnov; SP, stratum pyramidale; SO, stratum oriens.
| Cell name | TV58g | TV50a | MS14i | MS19b | AJ50j | AJ50h | AJ44j | MS74b | |
|---|---|---|---|---|---|---|---|---|---|
| Target hemisphere | Right | Right | Left | Left | Left | Right | Right | Right | |
| Varicosities per region (n sampled)* | Medial septum | 61 | u | 146 | n.o. | n.o. | 51 | n.o. | u |
| Dentate gyrus | n.o. | n.o. | n.o. | n.o. | n.o. | n.o. | n.o. | u | |
| CA3 | n.o. | n.o. | n.o. | n.o. | n.o. | n.o. | u | u | |
| CA2 | n.o. | n.o. | n.o. | n.o. | n.o. | n.o. | u | u | |
| CA1 | n.o. | n.o. | (c) | n.o. | n.o. | n.o. | u | u | |
| SUBd | 33 | n.o. | 3 | 104 | n.o. | c | u | u | |
| FC | 22 | (c) | n.o. | n.o. | n.o. | n.o. | u | u | |
| RSg | 85 (L6) | n.o. | n.o. | n.o. | 51 | n.o. | u | u | |
| Angular Bundle | 55 | 11 | n.o. | n.o. | n.o. | n.o. | u | u** | |
| PrSd L1-2 | n.o. | 3 | 8 | 15 | c | n.o. | u | u | |
| PrSd L3 | n.o. | 10 | 31 | 149 | n.o. | c | u | u | |
| PrSd L5-6 | 635 | 66 | 45 | 34 | 27 | 29 | u | u | |
| PaS | n.o. | n.o. | n.o. | n.o. | n.o. | n.o. | u | u | |
| EC | 695 | c | 32 | 63 | (c)*** | 6 | u | u | |
| Labeling strength | +++ | ++ | ++ | ++ | ++ | ++ | + | + | |
| Behavioral states | Max speed (cm/s) | u | u | 6.3 | 18.8 | 17.2 | 16.5 | 12.0 | u |
| Firing rate LM | 48.4 ± 6.0 | 51.4 ± 10.6 | 67.8 ± 12.9 | 43.4 ± 9.3 | 65.3 ± 11.7 | 33.6 ± 9.6 | 76.7 ± 11.0 | 55.0 ± 11.0 | |
| Firing rate SM | 41.6 ± 5.3 | 47.6 ± 8.8 | 51.0 ± 12.7 | 33.3 ± 8.6 | 42.5 ± 9.7 | 27.7 ± 3.8 | 43.8 ± 9.9 | u | |
| Firing rate WS | 47.1 ± 9.3 | u | 47.9 ± 9.0 | 33.2 ± 8.0 | 34.7 ± 6.1 | 24.3 ± 4.9 | 37.7 ± 2.5 | 34.2 ± 14.9 | |
| Firing rate IM | 44.2 ± 12.0 | 46.3 ± 10.5 | 48.2 ± 10.0 | 31.9 ± 5.2 | 46.6 ± 9.9 | 25.4 ± 7.6 | 38.1 ± 6.8 | 37.6 ± 16.0 | |
| Burst incidence LM | 5.0 ± 1.0 | 6.3 ± 0.9 | 3.8 ± 1.7 | 4.4 ± 0.9 | 6.7 ± 1.0 | 4.1 ± 1.7 | 6.2 ± 1.9 | 5.2 ± 1.3 | |
| Burst incidence SM | 4.4 ± 0.5 | 5.7 ± 1.0 | 4.7 ± 1.1 | 3.6 ± 1.1 | 5.5 ± 1.3 | 3.3 ± 0.8 | 5.6 ± 1.2 | u | |
| Burst incidence WS | 4.4 ± 0.9 | u | 4.6 ± 1.1 | 4.4 ± 1.3 | 4.6 ± 1.0 | 2.8 ± 0.8 | 6.0 ± 1.0 | 2.9 ± 1.4 | |
| Burst incidence IM | 4.2 ± 1.2 | 5.0 ± 0.9 | 4.9 ± 0.9 | 4.5 ± 1.3 | 5.4 ± 1.0 | 3.1 ± 1.3 | 5.3 ± 1.1 | 3.5 ± 0.9 | |
| CA1d theta (5 – 12 Hz) | LFP measurement | SO/SP | SP | upper SP | SP | SP | SP | SP | SP |
| Preferred theta phase | 159° | 176° | 152° | 167° | 177° | 264° | 130° | 233° | |
| Mean vector length (r) | 0.37 | 0.50 | 0.23 | 0.27 | 0.51 | 0.35 | 0.44 | 0.34 | |
| Rayleigh P-value | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | |
| Total n spikes | 1081 | 2679 | 6659 | 5143 | 1743 | 1413 | 860 | 578 | |
| Spikes per cycle (mean ± s.d.) | 6.8 ± 3.1 | 6.7 ± 3.0 | 8.7 ± 4.2 | 7.2 ± 3.4 | 7.2 ± 3.1 | 4.6 ± 1.9 | 7.7 ± 3.3 | 8.1 ± 3.2 | |