Figure 6.

Farnesol-induced cell death in TBY-2 suspension cells. A, Seven-day-old cells were subcultured (2–80 mL, dilution 41-fold) in the presence of different farnesol concentrations. After 5 d of culture the proportion of dead cells was determined. Cells were stained with fluorescein diacetate (specific for living cells) and propidium iodide (penetrates only into dead cells and stains nuclei orange-red). The percentage of dead cells corresponds to the proportion of red nuclei. Asterisks indicate values below 3% of dead cells, as was found in control cultures. B, Seven-day-old cells were subcultured by addition of 2, 5, 10, or 20 mL, respectively, to 80 mL of modified Murashige and Skoog medium (dilution factors of 41-, 17-, 9-, and 5-fold, respectively) containing 25 μm farnesol (final concentration). The percentage of dead cells was determined as in A.