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The scoring system used after IHC protocols to evaluate the density and the extent of the labelled deposits (brown; hematoxylin counterstaining, blue) by light microscopy: from minimum to maximum, for PrPsc (A–D); GFAP (E–H); and reactive microglia (I–L). Scale bars: 100 μm. GFAP = glial fibrillary acidic protein, IHC = immunohistochemistry, PrPsc = pathological prion protein.
