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Immunohistochemical patterns observed when each marker (brown; hematoxylin counterstaining, blue) was assessed. For PrPsc: (A) linear, (B) plaques, (C) spots, (D) coalescent, and (E) granular. Scale bars: 20 μm in (A); 50 μm in (B–E). For GFAP: (F) stellate, (G) perineuronal, and (H) radial. Scale bars: 50 μm in F and H; 20 μm in G. For reactive microglia: (I) ramified and (J) amoeboid. Scale bars: 20 μm. GFAP = glial fibrillary acidic protein, PrPsc = pathological prion protein.
