Figure 5.

Primary observations provided by light microscopic examination after the application of the IHC protocol for PrPsc detection (brown; hematoxylin counterstaining, blue). A, Purkinje cell layer and white matter from CJD cerebella always corresponded to the lowest intensity of protein deposit immunolabeling. B, Protein aggregates were not always associated with PrPsc immunostaining. C, PrPsc deposits were never observed in healthy controls. Scale bars: 50 μm in (A); 20 μm in (B); 200 μm in (C). CJD = Creutzfeldt–Jakob disease, IHC = immunohistochemistry, PrPsc = pathological prion protein, RT = room temperature.