Figure 7.

Primary observations provided by light microscopic examination after application of the IHC protocol for reactive microglia detection (brown; hematoxylin counterstaining, blue). A, An amoeboid morphology was found in a degenerated granular layer. B, Purkinje cells were not found to be in close association with reactive microglia. Ramified morphology was primarily observed in this cell layer. C, Meanwhile, amoeboid shapes were more frequently found in white matter. Scale bars: 50 μm in (A); 20 μm in (B) and (C). IHC = immunohistochemistry.