Steps	Reagents	Concentration
1. Mix reagents.	HEPES-KOH, pH 7.4	99 mM
NaCl	1.37 M
KCl	54 mM
NaH2PO4	1.7 mM
KH2PO4	2.2 mM
Glucose	33 mM
Sucrose	438 mM
autoclaved dH2O
2. Sterilize with 0.45 μm filter.
3. Store at 4 °C up to 6 months.
Note: For experimental use: dilute to 1X with autoclaved dH2O and sterilize with 0.45 μm filter top bottle by vacuum or with filter syringe. Keep at 4°C. Open in a culture hood, multiple use.