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. 2018 Jan 22;(131):55451. doi: 10.3791/55451
Amplicon length Amplification area Sequence Primer name Amplicon
332 - 405 bp Partial sequence of SSU, whole sequence of ITS1 and partial sequence of 5.8S  CGTAACAAGGTTTCCGTAGGTG SSU ITS1-flanks
GGTTCACGGGATTCTGCAAT 5.8S-R
318 - 361 bp Partial sequence of 5.8S, whole sequence of ITS2 and partial sequence of LSU ATTGCAGAATCCCGTGAACC 5.8S-F ITS2-flanks
TGCTTAAAYTCAGCGGGTAGYC LSU
100 - 200 bp ITS1 GGTATGGCGTCAAGGAACACT ITS1-F ITS1
ATAGCATCGCTGCAAGAGGT ITS1-R