Primer	Genus	Taxid ID	Species	Divergent primer
SSU	Arabidopsis	3701	kamchatica, thaliana and lyrata	-
Vicia	3904	villosa, americana, unijuga, amoenane, amurensis, craccamal, pseudo-orobus, multicaulis, japonica, ramuliflora and faba
Trifolium	3898	alexandrinum, montanum, resupinatum and repens	-
Nicotiana	4085	tabacum, benthamiana, otophora, picilla, bigelovii, palmeri, tomentosiformis, tomentosa, digluta, kawakamii, clevelandii, nesophila , solanifolia, cordifolia, debneyi, arentsii, thyrsiflora, wigandioides, undulata, glutinosa, noctiflora, petunioides, obtusifolia, miersii, pauciflora, attenuate, acuminata , linearis, alata, sylvestris , rustica and suaveolens	-
Cucumis	3655	anguria, melo and sativus	CGTAACAAGGTTTCCGTAGGKG
Aeluropus	110873	-	No primer found
Medicago	3877	sativa, lupulina, pamphylica, lunata, rostrate, plicata and truncatula	-
Oryza	4597	sativa, glumipatula, rufipogon barthii glaberrima punctate, longistaminata, meridionalis, nivara, meridionalis and longistaminata	-
Triticum	4564	aestivum, urartu and monococcum	-
Hordeum	4512	vulgare, bulbosum, marinum, brevisubulatum and bogdanii	-
LSU	Arabidopsis	3701	petraea, thaliana and lyrata	TGCTTAAACTCAGCGGGTAATC
Vicia	3904	sylvatica, tetrasperma, sativa,hirsute, sepium, parviflora, cracca, lathyroides, orobus, orobus,bithynica and faba	TGCTTAAATTCAGCGGGTAGCC
Trifolium	3898	pretense, nigrescens, resupinatum, occidentale,subterraneum,strictum,ochroleucon, glomeratum,squamosum, ornithopodioides and repens	TGCTTAAATTCAGCGGGTAGCC
Nicotiana	4085	tabacum, benthamiana, otophora, picilla, bigelovii, palmeri, tomentosiformis, tomentosa, digluta, kawakamii, clevelandii, nesophila , solanifolia, cordifolia, debneyi, arentsii, thyrsiflora, wigandioides, undulata, glutinosa, noctiflora, petunioides, obtusifolia, miersii, pauciflora, attenuate, acuminata , linearis, alata, sylvestris and suaveolens	TGCTTAAACTCAGCGGGTAGTC
Cucumis	3655	melo, ritchiei and javanica	TGCTTAAACTCAGCGGGTAGTC
Aeluropus	110873	lagopoide, pungens and littoralis	TGCTTAAATTCAGCGGGTAATC
Medicago	3877	ruthenica, sativa, lupulina, arabica, polymorpha and minima	TGCTTAAATTCAGCGGGTAGCC
pamphylica, lunata, rostrate and plicata	TGCTTAAACTCAGCGGGTAGTC
Oryza	4597	sativa,glumipatula, rufipogon,barthiial, glaberrima,australiensis,officinalis, australiensis, ridleyi, malampuzhaensis , alta, nivara, rufipogon, meridionalis and longistaminata	TGCTTAAACTCAGCGGGTAGTC
Triticum	4564	aestivum,spelta, turgidum,dicoccoides, petropavlovskyi, urartu and monococcum	TGCTTAAACTCAGCGGGTAGTC
Hordeum	4512	vulgare, bulbosum, murinum,secalinum, brevisubulatum and bogdanii	TGCTTAAACTCAGCGGGTAGTC
A degenerate primer is defined as IUPAC system for nucleotide nomenclature