| Techniques for analysis | Potential implications |
| Quantitative Real-Time Polymerase Chain Reaction | Investigate collagen markers (Col I, Col III, and Col V), fibrotic marker alpha-Smooth muscle actin (α-SMA), and key mediators in DM including Insulin growth factor-1 (IGF-1) and it’s receptor (IGF1-R) |
| Transmission Electron Microscopy | Structural changes are investigated such as collagen fibrils and cell-ECM interactions |
| Immunofluorescence | Use of specific antibodies chemically conjugated to fluorescent dye to investigate cellular antigens |
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