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. Author manuscript; available in PMC: 2019 Apr 19.
Published in final edited form as: Chem Commun (Camb). 2018 Apr 19;54(33):4172–4175. doi: 10.1039/c8cc01639a

Fig. 2.

Fig. 2

Genetic incorporation of N6-((2-(vinylthio)ethoxy)carbonyl)-L-lysine (VtK). (A) The structure of VtK; (B) SDS-PAGE analysis of the expression of a myoglobin mutant (Mb-Lys99TAG) with and without VtK; (C) Fluorescence readings of cells expressing VtKRS-1 and sfGFP-Asn149TAG mutant. Fluorescence intensity was normalized to cell growth. All protein expressions were conducted either in the presence or in the absence of 1.0 mM VtK.