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Small molecule labeling of protein through the oxidation-induced Michael addition reaction. Following labelling reactions, protein samples (wild-type sfGFP or sfGFP-Asn149VtK mutant) were denatured by heating, then analyzed by SDS-PAGE. The top panel shows Coomassie blue stained gel and the bottom panel shows the fluorescent image of the same gel before Coomassie blue treatment. DNSEDA, N-dansylethylene-diamine.
