Fig. 4.
Crosslinking of GST dimer through the oxidation-induced Michael addition reaction. All GST samples (~50 μM) were incubated in the presence of 5 mM H2O2 and 0.5 mM Na2WO4 at 37 °C for 60 min. TCEP (10 mM) was added after the oxidation reaction before SDS-PAGE analysis.