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. 2018 Apr;109(4):728–734.e2. doi: 10.1016/j.fertnstert.2017.12.024

Figure 1.

Figure 1

Dehydroepiandrosterone (DHEA) increases androgen synthesis during decidualization. Human endometrial stromal fibroblasts (HESFs) were treated with either vehicle control (VC), decidualization (DEC) media, or DEC media supplemented with 10 nM DHEA (DEC DHEA) (A, B) or DEC media supplemented with either 10 or 100 nM DHEA (C, D). (A, B) Secretion of both T and dihydrotestosterone (DHT) was detected in DEC groups at all time points and significantly increased in DEC DHEA (supplemented with 10 nM DHEA) compared with DEC alone. Neither T nor DHT was detected in hESFs treated with VC (not shown). (C, D) HESFs were decidualized for 4 days and supplemented with 10 nM or 100 nM DHEA. T and DHT were significantly increased in a dose-dependent manner with increasing DHEA concentrations. Dotted lines show circulating concentrations of T in women (Table 1). n = 6–16 patients, duplicate treatments. Two-way ANOVA (A, B) or Kruskal-Wallis test (C, D). ***P<.001; ****P<.0001.