Fig. 3.

Pellino2 is required for in vivo expression of IL-1β and lethality in response to LPS. ELISA of a IL-6, b TNF, c CXCL1, d RANTES, and e IL-1β, from serum of WT and Peli2^−/− mice at 16 h post-intraperitoneal injection with 20 mg/kg LPS or PBS (n = 7–12). f Survival rates of age- and sex-matched WT (n = 12) and Peli2^−/− (n = 12) mice after intraperitoneal injection with 50 mg/kg of LPS or PBS (n = 4 for each of WT and Peli2^−/−) as a vehicle control. Mice were monitored every 6 h for 72 h. **P < 0.01 (paired, two-tailed Student’s t-test (e) and log-rank Mantel-Cox test (f)). Data indicate samples from individual mice (n = 4 for all PBS groups (a–f), n = 9 for LPS groups (a), n = 8 for LPS/WT, n = 9 for LPS/ Peli2^−/− (b), n = 7 for LPS groups (c, d), n = 11 for LPS/WT, n = 12 for LPS/Peli2^−/− (e). Data are pooled biological replicates from two independent experiments. Error bars, s.e.m.