Fig. 4.

Pellino2 mediates activation of the NLRP3 pathway in response to bacterial infection. a-f WT and Peli2^−/− BMDMs were infected with a, b C. rodentium, c, d E.coli, or e, f P. aeruginosa (PA01 strain) at a multiplicity of infection (MOI) of 100. a, c, e ELISA of IL-1β, IL-18, and CXCL1 in medium from BMDMs infected for 6 h. b, d Immunoblot analysis of Caspase-11 in lysates from cells infected for 0–6 h. f Immunoblot analysis of IL-1β and Caspase-1 in lysates from cells infected with PAO1 for 3 h. β-Actin was used as loading controls. g ELISA of IL-1β, IL-18, and IL-6 in peritoneal lavage from WT and Peli2^−/− mice previously infected for 10 h by intraperitoneal injection of PAO1 (1.5 × 10⁷ CFU). *p < 0.05, **p < 0.01 (paired, two-tailed Student’s t-test). Data represent the mean ± s.e.m. of three independent experiments (a, c, e), biological replicates that are representative of 3 independent experiments (b, d, f), or samples from individual mice (n = 4–6 per group) (g)