Table 2.
Primers used for RT-PCR amplification and sequencing of goose astrovirus isolate AAstV/Goose/CHN/2017/SD01
| Primer name | Sequence (5′ → 3′) | Location in genome | Product size (bp) |
|---|---|---|---|
| GAstV5race | TCGTTTCAGCGAGCATTGTAGCCTCTGT | 385–414 | 414 |
| GAstV F1 | AAAACAGCAATTGGCGTTGA | 236–255 | |
| GAstV R1 | TTTTTCCCTCAACRATRACAAT | 1667–1688 | 1453 |
| GAstV F2 | TGTGGTCTACAGCCTTGA | 1404–1418 | |
| GAstV R2 | TCAACTTGTTCATCCTTTAC | 2792–2811 | 1408 |
| GAstV F3 | AGATTGATGAAGCCATTGAG | 2604–2624 | |
| GAstV R3 | TGCCGACGCTCAGATT | 4340–4355 | 1752 |
| GAstV F4 | ACCATCATAAGACACCACAG | 4127–4146 | |
| GAstV R4 | TCATTTTGTCATTAACGGG | 5014–5032 | 906 |
| GAstV F5 | GGGCGGTGGCCCCGCGCG | 4835–4852 | |
| GAstV R5 | CTTGACCTGGATTCTGCC | 6186–6203 | 1369 |
| GAstV F6 | TACTCCCTCGCTTGTGTACA | 6102–6121 | |
| GAstV R6 | CTCGGCGTGGCCKCSRCTGCTG | 6952–6970 | 869 |
| GAstV3race | TTGGTCAGTGTCAGATTCC | 6630–6648 | 546 |
| GAstVF7 | ATTCTTGGCTCGGTTGTC | 5239–5256 | |
| GAstVR7 | CCTGTGTTGCTCCTTCTC | 5710–5727 | 489 |