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. 2018 Apr 19;9:1555. doi: 10.1038/s41467-018-04025-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Protein subcellular localization and recovery of P. patens clones after dehydration treatments. a YAN is localized in oil bodies as indicated by the green fluorescence of YAN-GFP merged with Nile red and OLEOSIN-RFP (red), respectively. AltYAN-GFP is localized in chloroplasts as shown by the merged green fluorescence of AltYAN-GFP with chlorophyll auto-fluorescence (red). Scale bar = 4 μm. b Expression profile of YAN/AltYAN under dehydration and rehydration. Results represent means and standard deviations of three biological replicates for the wild-type plants. c Phenotypes of wild-type plants and yan/altyan mutants under control and dehydration treatments. Wild-type and yan/altyan plants were dehydrated for 20 h and transferred into sterile water for 1 h, and then transferred onto standard medium for recovery. Photos were taken after plants were recovered for 2 weeks. Scale bar = 2 mm. d Chlorophyll contents of wild-type and yan/altyan plants after 2 weeks of recovery. Results represent means and standard deviations of three biological replicates for the wild-type and two independent mutants. Double asterisks indicate a statistically significant difference compared with wild-type plants based on a two-tailed Student’s t test (p < 0.01)