Fig. 4.

In situ oil bodies and their quantification. a Accumulation of oil bodies in wild-type plants and yan/altyan mutants. Four-week old plants were dehydrated for 30 min, and plants under normal growth conditions were used as control. Plants were stained with Nile red dye (25 μg ml⁻¹ methanol) for 12 h before observation. Scale bar = 20 μm. b Quantification of oil bodies in wild-type plants and yan/altyan mutants under dehydration treatments. Results represent means and standard deviations of three biological replicates for the wild-type and yan/altyan-8#. Double asterisks indicate a statistically significant difference compared with wild-type plants based on a two-tailed Student’s t test (p < 0.01). c YAN-OE and AltYAN-OE plants accumulated excessive oil bodies under normal conditions and showed delayed growth. Two-week old wild-type as well as YAN-OE and AltYAN-OE plants were used to observe oil bodies. Scale bar = 0.2 mm