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. 2018 Apr 19;8:6290. doi: 10.1038/s41598-018-24664-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of enzyme-catalyzed Ag growth on Au nanoparticle-assembled structures. In the presence of target IgG, the immune complex Ab₁-IgG-Ab₂, which contains alkaline phosphatase (AP), triggers enzyme-catalyzed conversion of 2-phospho-L-ascorbic acid to ascorbic acid. The ascorbic acid reduces AgNO₃ to Ag metal which can be deposited on the surface of AuNPs on silica NPs. As Ag deposition on the surface of SiO₂@Au results in strong absorbance, significant colorimetric changes can be observed, allowing for highly sensitive colorimetric immunoassay.