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. 2018 Apr 19;8:6277. doi: 10.1038/s41598-018-24749-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hyperthermia inhibited IL-6 mRNA and production in HCFs. (a) IL-6 mRNA expression in HCFs stimulated by hyperthermia (42 °C) and/or TGF-β1 for 6 h (n = 4, ***p < 0.001). (b) IL-6 production from HCFs stimulated by hyperthermia (42 °C) and/or TGF-β1 for 24 h (n = 4, ***p < 0.001, ns: no significant difference). (c) Time dependence of the effect of hyperthermia (42 °C) effect on IL-6production in the presence of TGF-β1 for 24 h (n = 4–5, **p < 0.01, ***p < 0.001). (d) Effects of hyperthermia (42 °C) and mild hyperthermia (39 °C) on IL-6 production in the presence of TGF-β1 for 24 h (n = 4–8, *p < 0.05, **p < 0.01, ***p < 0.001).