Figure 2.

Outcome on inflammation and arthritis pathology following adenovirally overexpressing Pros1 in KRN serum transfer arthritis (STA). (A) Bone marrow-derived macrophages were transduced with Ad Luc or Ad Pros1 and stimulated with lipopolysaccharide (LPS) or P3C for 6 (top) or 24 h (bottom). Messenger RNA was extracted and gene expression was determined (top). Supernatants were analyzed (bottom). Data are representative for two independent experiments (n = 3 per experiment). (B) KRN STA was induced in mice intra-articularly injected with Ad Luc or Ad Pros1 (n = 9 mice). Knee synovial biopsies were obtained, mRNA was extracted and gene expression was determined (n = 6 knee synovial biopsies). (C) Shown are representative pictures of knee joint sections stained with safranin O and fast green. (D) Histological knee joint sections were scored for the arthritis parameters depicted. Three semi-serial sections per joint were scored in a random and blinded manner with an arbitrary score from 0 to 3 (n = 12 knee joints). P, patella; F, femur; S, synovium; JC, joint cavity. For (A), data are presented as dot-plots with mean. For (B–D), data are represented as mean + SEM. *p < 0.05, **p < 0.01, ***p < 0.001 with unpaired t-test. See also Figures S1 and S2 in Supplementary Material.