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. 2018 Apr 13;9:742. doi: 10.3389/fimmu.2018.00742

Figure 4.

Figure 4

Effect of MER-specific antibody treatment on clinical severity and pathology of collagen-induced arthritis (CIA). (A) Mice with CIA were intravenously injected with IgG or anti-MER (n = 11–12 mice). Two mice were euthanized 1 h after injection. Lung, liver, and spleen were isolated and samples were immunoprecipitated (IP) for MER and immunoblotted for MER or phosphorylation (pY) (n = 1). (B) Macroscopic score of knee joints as determined by swelling and blood vessel formation (n = 20–22 knee joints). (C) Shown are representative pictures of histological knee joint sections stained with safranin O and fast green of each group. (D) Histological knee joint sections were scored for the arthritis parameters depicted. Three semi-serial sections per joint were scored in a random and blinded manner with an arbitrary score from 0 to 3 (n = 20–22 knee joints). P, patella; F, femur; S, synovium; JC, joint cavity. For (B–D), data are presented as mean + SEM. *p < 0.05, **p < 0.01 with unpaired t-test. See also Figure S4 in Supplementary Material.