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. 2018 Apr 13;9:789. doi: 10.3389/fimmu.2018.00789

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Copyright © 2018 Jiao, Song, Huang, Xiang, Cui, Wu, Qu, Wang, Ouyang and Liao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The hemagglutination inhibition (HI) antibody titers of infected and contacted chickens. Serum samples were collected from CK134-inoculated chickens and naïve chickens in the same isolator, and all of the samples were analyzed by HI tests. An HI titer >2³ was considered indicative of the CK134 virus, whereas titers <2³ were considered negative (12). Statistical analysis was performed using the two-way ANOVA test (GraphPad Prism 7.0 software), p < 0.05 was considered statistically significant (*p < 0.05; **p < 0.01).