Figure 5.

Embryogenic frequency as a function of Ca²⁺/pharmacological agents in MS₃. A, Percentage embryogenesis as a function of Ca²⁺ concentration in the differentiation medium; 0 mm Ca²⁺ was obtained by the addition of 1 mm EGTA into MS₃ lacking CaCl₂. B, Percentage embryogenesis as a function of increasing concentrations of the Ca²⁺-channel blockers, namely nifedipine, bepridil, and verapamil. C, Percentage embryogenesis as a function of increasing concentrations of W7. D, Percentage embryogenesis obtained with increasing concentration of the Ca²⁺ ionophore A23187. Percentage embryogenesis in the different culture treatments was calculated relative to the 100% embryogenesis obtained in unmodified MS₃ or the DMSO control both containing 3.9 mm Ca²⁺.