Figure 6.

Embryo development under different culture treatments. A, Control showing bipolar- and torpedo-stage embryos emerging from embryogenic cell clumps after d 21 culture incubation; inset a, PEM as seen in the initial inoculum; B, enlarged cell clumps grown in MS₃ medium containing 1 mm EGTA; C, enlarged cell clumps obtained in MS₃ medium containing 500 μm nifedipine; D, fused embryos under Ca²⁺ ionophore treatment; E, enlarged and fused embryos under W7 treatment. Scale bar = 15 μm (inset a); = 6 mm (A–C), = 2 mm (D and E).