Figure 9.

CDPK activity and swCDPK protein in soluble protein extracts of embryogenic cultures subjected to treatments with different pharmacological agents. A, Ca²⁺-Dependent histone phosphorylation activity in embryogenic cultures grown under optimal differentiation conditions (Con), Ca²⁺-chelated conditions (EGTA), 100 μm Ca²⁺ ionophore (I), 500 μm nifedipine (Nif), 500 μm verapamil (Verp), and 500 μm bepridil (Bep) treatments. B, Ca²⁺-Dependent phosphorylation of the 55-kD swCDPK protein determined by assaying in the absence of exogenous substrate followed by SDS-PAGE. C, Immunodetection of the 55-kD swCDPK band using polyclonal antisoybean CDPK in soluble proteins from cultures grown under optimal conditions and different pharmacological treatments.