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. 2018 Apr 19;8:6292. doi: 10.1038/s41598-018-24573-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bray-Curtis dissimilarity between observed and expected strain proportion for each of the tested extraction methods. The expected strain proportion (actual) was generated for comparison and represents the theoretically composition of the mock community (see methods for detail). The pair-wise proportions (expected to observed) from samples extracted with the same commercial kit were grouped in a single boxplot (mean ± SEM). Symbols illustrate different buffer types (■ PBS, ▼RNA-later, ▲native, ●lysis buffer) (Paired t-test, *p < 0.05, ***p < 0.001).