Table 1.
Overview about investigated strains with respect to strain-number, taxonomic class and several indicators of cell wall resistance: percentage of Crystal Violet (CV), Ruthenium red (RR) and Toluidine blue O (TbO) stained cells, Calcofluor/Forward Scatter staining index (CF/FSC-SI), mean p-adjusted R-value of a linear model for high-density region over 5 days (CF/FSC-Corr.) and references of published algaenan-containing strains.
| Strain | Class | Algaenan | CV stained cells | RR stained cells | TbO stained cells | CF/FSC-SI | CF/FSC-Corr. | Definition |
|---|---|---|---|---|---|---|---|---|
|
Acutodesmus obliquus (SAG 276-3a) |
Chlorophyceae | Yes [UTEX 1450 (Kodner et al., 2009); Strain 633 (Zych et al., 2009)] |
25% | 0% | 0% | 0.1 ± 0.0 | 0.0 ± 0.0 | HR |
|
Desmodesmus armatus (SAG 276-4 d) |
Chlorophyceae | Yes (Allard and Templier, 2000) | 16% | 0% | 30% | 0.1 ± 0.0 | 0.1 ± 0.0 | HR |
|
Chlorella vulgaris (SAG 211-11b) |
Trebouxiophyceae | No [Strain 136 (Zych et al., 2009)] | 100% | 8% | 0% | 0.7 ± 0.1 | 0.5 ± 0.1 | MR |
|
Oocystis marssonii (SAG 257-1) |
Trebouxiophyceae | No (Robinson, 1981) | 100% | 0% | 0% | 2.3 ± 0.2 | 0.6 ± 0.1 | LR |
|
Chloroidium saccharophilum (SAG 211-9a) |
Trebouxiophyceae | No (Burczyk et al., 1999; Zych et al., 2009) |
100% | 0% | 0% | 2.2 ± 0.1 | 0.4 ± 0.0 | LR |
By consideration of all different indicators for cell wall resistance, the investigated strains were divided in highly resistant (HR), medium resistant (MR) and low resistant (LR) species.