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. 2018 Apr 13;11:130. doi: 10.3389/fnmol.2018.00130

Figure 3.

Figure 3

FN upregulated proliferation of GSCs. (A) After U87-GSCs were cultured for 72 h, cells were shown to proliferate when grown on 5 or 10 μg/mL FN. (B) Immunofluorescence staining revealed FN at 5 or 10 μg/mL induced increased expression of Ki-67 by primary-GSCs, indicating FN promoted cell proliferation. Whereas primary-GSCs were detached by cilengitide and Ki67 were decreased markedly comparing to that in the 10 μg/mL FN group. Images were taken at the same exposure settings. Cells grown without FN and stained with isotype control Mouse IG1 were used as a negative control. (C–E) Western blots showed the marked upregulation of p-ERK1/2 and cyclin D1 by U87-GSCs grown on 5 or 10 μg/mL FN. Primary-GSCs showed markedly higher expression of these two proteins when grown on 10 μg/mL FN, with or without carmustine treatment. Cilengitide significantly suppressed both p-ERK1/2 and cyclin D1 expression of U87-GSCs. However, cilengitide only suppressed cyclin D1 expression in primary-GSCs. *p < 0.05, n.s. not significant.