Table 2.

PCR primers and probes used in this study.

Name	Description	Sequence (5′–3′)	Product size (bp)
509CF	Forward primer – dPCR S509T assay	GTCCCTCTTCTCCCATGCAAT	73
509CR	Reverse primer – dPCR S509T assay	TGCCGAAACGGATTACTCAAG	73
S509 Probe	dPCR probe S509	Calorange560-AGTATGTTTTCTCGGCCAA-BHQ1	NA
T509 Probe	dPCR probe T509	FAM-AGTATGTTTACTCGGCCAA-BHQ1	NA
136AF	Forward primer – dPCR Y136F assay	ATGCCGAAGAAATTTATACG	150
136AR	Reverse primer – dPCR Y136 assay	GAACTGTGCAAATATCAGAG	150
Y136 Probe	dPCR probe Y136	FAM-AGGACAGTCAAACACTACA-BHQ1	NA
F136 Probe	dPCR probe F136	Calorange560-AGGACAGTCATACACTACA-BHQ1	NA
Bgh51_1F	Cyp51 gene sequencing primer	TAGACTTCCATTTTCCGTCCT	736
Bgh51_1R	Cyp51 gene sequencing primer	GGGTGTGTGAAGCAGTGTATATCGT	736
Bgh51_2F	Cyp51 gene sequencing primer	TATCGATGCAGTAATGGCTGA	700
Bgh51_2R	Cyp51 gene sequencing primer	AGTGTCCCAACGATGTGGAT	700
Bgh51_3F	Cyp51 gene sequencing primer	AGTAAAGAATCCAATGCCCGT	528
Bgh51_3R	Cyp51 gene sequencing primer	CATCAATTGGCAGGTAGTGA	528
BghCYP509F	Amplicon sequencing forward primer	CCACCATGGTTCGCAGTTTC	280
BghCYP509R	Amplicon sequencing reverse primer	TGGTAGCTACGGTCCAGTCA	280

Nucleotides shown in bold are locked nucleic acids (LNA). BHQ1, Black hole quencher 1.