Figure 2.

AAV vectors transduced diverse inner-ear cell types in adult cochleae. Two weeks after injection via canalostomy, whole-mount immunofluorescence showed AAV infection in the sensory and non-sensory cells from the apical turn of the cochlea. Hair cells were labeled with MYO7A (red). (A–C) AAV2 infected most IHCs and some outer hair cells (OHCs). (D–F) AAV2/Anc80L65 infected most IHCs as well as most OHCs. (G–I) AAV8 infected most IHCs, with weak expression in some OHCs. (J–L) AAV9 infection was limited to some IHCs. Scale bars: 50 μm.