Figure 7.

AAV transduction by canalostomy in adults does not impair HC survival and maintains transgene expression long term. (A–F) Fifty-one days after AAV2/Anc80L65-GFP injection, no significant HC loss was detected in the middle turn of the injected (A–C) compared to the uninjected ear (D–F). Arrows indicate sporadic and weak GFP⁺ IHCs in the uninjected ear, likely due to migration of viral particles from the injected ear. Green, GFP; red, MYO7A. (G–J) there is no significant difference in total number of HCs (G and H) or HC loss (I and J) between injected and uninjected ears. n = 4. Scale bars: 50 μm.