Table 1.
Efficiency of HC infection by AAV injection through canalostomy
| Number/percentage of GFP+ HCs by frequencies (kHz) | ||||||||
|---|---|---|---|---|---|---|---|---|
| Serotype | Cell type | 5.66 | 8 | 11.32 | 16 | 22.64 | 32 | 45.25 |
| AAV1 | IHC | 2.8 ± 0.4 | 2.1 ± 0.5 | 1.2 ± 0.3 | 3.2 ± 1.3 | 1.8 ± 0.2 | 1.9 ± 0.2 | 1.3 ± 0.3 |
| 10.4 ± 1.3% | 7.9 ± 2.0% | 4.4 ± 1.0% | 11.8 ± 4.7% | 6.6 ± 0.9% | 7.0 ± 0.6% | 4.9 ± 1.2% | ||
| OHC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| AAV2 | IHC | 24.2 ± 4.9 | 25.8 ± 2.2 | 26.7 ± 0.5 | 23.4 ± 0.5 | 25.5 ± 1.5 | 23.1 ± 4.6 | 13.1 ± 10.0 |
| 89.6 ± 18.0% | 95.4 ± 8.0% | 98.9 ± 1.9% | 86.5 ± 13.9% | 94.4 ± 5.7% | 85.5 ± 17.0% | 48.5 ± 36.9% | ||
| OHC | 16.6 ± 13.0 | 9.8 ± 10.0 | 8.7 ± 7.9 | 5.8 ± 2.4 | 6.8 ± 6.2 | 1.3 ± 1.3 | 0 | |
| 20.5 ± 16.0% | 12.1 ± 12.3% | 10.8 ± 9.7% | 7.1 ± 3.0% | 8.4 ± 7.7% | 1.6 ± 1.6% | 0.1 ± 0.2% | ||
| AAV2/Anc80L65 | IHC | 26.4 ± 1.0 | 26.6 ± 0.7 | 26.0 ± 0.9 | 26.6 ± 0.7 | 21.9 ± 8.5 | 24.1 ± 5.0 | 22.7 ± 4.1 |
| 97.8 ± 3.8% | 98.4 ± 2.7% | 96.3 ± 3.2% | 98.4 ± 2.7% | 81.1 ± 31.3% | 89.3 ± 18.5% | 84.1 ± 15.1% | ||
| OHC | 57.4 ± 23.5 | 54.4 ± 23.1 | 54.8 ± 25.1 | 32.2 ± 25.7 | 12.3 ± 10.9 | 8.4 ± 14.7 | 2.0 ± 3.5 | |
| 70.9 ± 29.8% | 67.2 ± 28.5% | 67.7 ± 31.0% | 39.7 ± 31.7% | 15.2 ± 13.4% | 10.4 ± 18.1% | 2.5 ± 4.3% | ||
| AAV6.2 | IHC | 2.6 ± 0.4 | 3.2 ± 0.5 | 1.2 ± 0.3 | 0.8 ± 0.2 | 1.1 ± 0.2 | 0.7 ± 0.2 | 0.9 ± 0.2 |
| 9.6 ± 1.3% | 11.9 ± 1.7% | 4.4 ± 1.0% | 3.1 ± 0.8% | 4.0 ± 0.9% | 2.6 ± 0.6% | 3.3 ± 0.7% | ||
| OHC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| AAV8 | IHC | 16.8 ± 2.1 | 16.5 ± 5.1 | 19.4 ± 4.6 | 21.7 ± 3.4 | 24.9 ± 5.0 | 19.6 ± 7.4 | 17.8 ± 13.4 |
| 62.2 ± 7.7% | 61.0 ± 18.9% | 71.7 ± 16.9% | 80.3 ± 12.7% | 92.4 ± 18.6% | 72.6 ± 27.5% | 65.9 ± 49.7% | ||
| OHC | 1.5 ± 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | |
| 5.5 ± 6.1% | 0 | 0 | 0 | 0 | 0 | 0 | ||
| AAV9 | IHC | 16.8 ± 2.2 | 15.9 ± 2.1 | 16.4 ± 3.2 | 16.7 ± 5.1 | 24.1 ± 5.0 | 13.3 ± 3.2 | 15.4 ± 8.3 |
| 62.2% ± 8.0% | 58.8% ± 7.6% | 60.6% ± 11.8% | 61.8% ± 19.0% | 89.4 ± 18.6% | 49.3 ± 11.9% | 56.9 ± 30.7% | ||
| OHC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| AAVrh.39 | IHC | 15.0 ± 4.2 | 11.4 ± 3.2 | 20.3 ± 3.2 | 9.8 ± 1.3 | 17.8 ± 2.0 | 16.5 ± 2.1 | 9.9 ± 4.6 |
| 55.6 ± 15.7% | 42.3 ± 12.0% | 75.0 ± 11.8% | 36.2 ± 4.7% | 65.9 ± 7.5% | 61.1 ± 7.9% | 36.6 ± 17.2% | ||
| OHC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| AAVrh.43 | IHC | 26.8 ± 0.4 | 25.2 ± 0.8 | 25.5 ± 2.2 | 25.0 ± 1.7 | 25.4 ± 1.8 | 25.5 ± 1.1 | 25.8 ± 1.5 |
| 99.2 ± 1.4% | 93.2 ± 3.1% | 94.4 ± 8.2% | 92.7 ± 6.1% | 94.2 ± 6.4% | 94.3 ± 3.9% | 95.6 ± 5.6% | ||
| OHC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
The infected cells are indicated by the number per 200 μm as well as by the percentage of all respective HCs.
HC, hair cells; AAV, adeno-associated virus; GFP, green fluorescent protein; IHC, inner hair cells; OHC, outer hair cells.