FIG. 6.

Phenotypic characterization of cells in the pulmonary vasculature of rhesus macaques infected with SHIVnef. Immunofluorescent staining for smooth muscle actin (yellow, A), macrophages (CD163 in yellow, B), proliferative cells (Ki-67 in yellow, C), stem cells (CD34 in yellow, D), and T lymphocytes (CD3 in yellow, E). Each staining was multiplexed with staining for endothelial cells (Von Willebrand factor, green), Nef (red), and nuclei (blue). Macaques with statistically significant higher immunofluorescence are shown (see Fig. 6 for quantification results); macaque ID and weeks postinfection (WPI) are shown on top of each panel. The insets indicated by rectangles on the left are shown to the right, in higher magnification. Panel F shows the isotype controls (mouse IgG, yellow) multiplexed with staining for endothelial cells and nuclei. All figures show merged images. Magnifications are shown for each figure.