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. 2018 Apr 11;3(2):e00095-18. doi: 10.1128/mSphere.00095-18

FIG 5.

FIG 5

Decreased antibiotic susceptibility of E. coli efflux pump overexpression strain FL15. (A) pZE-acrAB-tolC vector for AcrAB-TolC efflux pump overexpression. The tolC gene, together with its RBS, is placed downstream of the acrAB operon so that the three genes form a single cistron. AcrAB-TolC efflux pump expression is driven by the Ptac promoter. The vector contains the placIIq-lacI cassette for regulation of Ptac promoter expression. (B) Growth rate of E. coli efflux pump overexpression strain FL15 (acrB-deficient strain harboring pZE-acrAB-tolC) and control strain FL30 (acrB-deficient strain harboring pZE-gfp) upon tetracycline treatment under normal conditions (glucose medium containing 0.1 M NaCl). The data obtained with the wild-type (WT) strain at both 0.1 and 0.4 M NaCl are plotted together for comparison. (C) Growth rates of E. coli FL15 and FL30 strains upon chloramphenicol treatment under normal conditions (glucose medium containing 0.1 M NaCl). The data obtained with the wild-type strain at both 0.1 and 0.4 M NaCl are plotted together for comparison.