Fig. 1. Feasibility analysis of the THP-AuNR nanoprobe. (A) Ideal structure and thermal kinetic parameters of HDP. (B) Ideal structure and thermal kinetic parameters of HAP. (C) Verification of the experimental design by gel electrophoresis: lane 1: HDP (1 μM); lane 2: HAP (1 μM); lane 3: miRNA-373 (1 μM); lane 4: HDP (1 μM) and HAP (1 μM); lane 5: HAP (1 μM) and miRNA-373 (1 μM); lane 6: HDP (1 μM) and miRNA-373 (1 μM); lane 7: HDP (1 μM), HAP (1 μM) and miRNA-373 (1 μM). (D) Gel electrophoresis amplified assays: HDP (1 μM), HAP (1 μM) and miRNA-373. Lanes 1–7: 1 μM, 100 nM, 10 nM, 1 nM, 100 pM, 10 pM, and 1 pM of miRNA-373, respectively.
