Table 1. Binding Behavior of Gal-1 and Gal-3 Using Multivalent TDG Conjugates as Immobilized Ligands.

galectin	ligand	apparent Kd (μM)a	Bmax (−)a	galectin binding efficiency [μM–1]b
Gal-1	11	0.090 ± 0.012	0.37 ± 0.01	4.1 ± 0.8
Gal-1	12	0.131 ± 0.017	0.43 ± 0.01	3.3 ± 0.6
Gal-3	11	0.616 ± 0.246	0.86 ± 0.12	1.4 ± 0.5
Gal-3	12	0.199 ± 0.045	1.04 ± 0.06	5.2 ± 0.3

^aDetermined in ELISA.

^bRatio of B_max and apparent K_d.