Table 2.
Primers used for Real-time PCR
| Genes | Sequence (5’to3’) | Product size, bp | GenBank No. |
|---|---|---|---|
| GPX4 | F: CACCCTCTGTGGAAGTGGAT | 112 | NM_214407.1 |
| R: TCACCACACAGCCGTTCTTA | |||
| GPX1 | F: AAATGCTCACCCGCTCTTC | 118 | NM_214201.1 |
| R: GTCATTGCGACACACTGGAG | |||
| CAT | F: ACGCCTGTGTGAGAACATTG | 124 | NM_214301.2 |
| R: GTCCAGAAGAGCCTGAATGC | |||
| SOD3 | F: ACGCTGCTCTGTGCTTACCT | 135 | NM_001078688.1 |
| R: CTGCCAGATCTCCGTCACTT | |||
| SOD2 | F: TGGAGGCCACATCAATCATA | 113 | NM_214127.2 |
| R: TTTCGAAGGAACCAAAGTCG | |||
| SOD1 | F: TCCATGTCCATCAGTTTGGA | 131 | NM_001190422.1 |
| R: AGTCACATTGCCCAGGTCTC | |||
| GAPDH | F: ATGGTGAAGGTCGGAGTGAA | 155 | NM_001206359.1 |
| R: CCGTGGGTGGAATCATACTG | |||
| NQO1 | F: CCAGCAGCCCGGCCAATCTG | 160 | NM_001159613.1 |
| R: AGGTCCGACACGGCGACCTC | |||
| HO1 | F: AGGCTGAGAATGCCGAGTTC | 90 | NM_001004027.1 |
| R: TGTGGTACAAGGACGCCATC | |||
| CYP1A1 | F: CTGCCATCTTCTGCCTTGTA | 314 | NM_214412.1 |
| R: GCTCTGGCCATTAGAGATCA | |||
| TXNRD1 | F: CTTTACCTTATTGCCCGGGT | 162 | NM_214154.2 |
| R: GTTCACCGATTTTGTTGGCC | |||
| GCLM | F: CTTGCCTCTTGCTGTGTGAT | 159 | XM_001926378.4 |
| R: CCACTCATGTGCCTCGATGT | |||
| MGST1 | F: TTGGCGCGCGAATCTACCACA | 239 | NM_214300.1 |
| R: TCCTCGGCTCCCTTCCCACTTA | |||
| UGT1A1 | F: GATCCTTTCCTGCAACGCAT | 313 | XM_001927673 |
| R: GGAAGGTCATGTGATCTGAG | |||
| IL-6 | F: AGCAAGGAGGTACTGGCAGA | 257 | NM_001252429.1 |
| R: GTGGTGGCTTTGTCTGGATT | |||
| IL-8 | F: ACTTCCAAACTGGCTGTTGC | 120 | NM_213867.1 |
| R: GGAATGCGTATTTATGCACTGG | |||
| IL-1β | F: GTTCTCTGAGAAATGGGAGC | 143 | NM_214055.1 |
| R: CTGGTCATCATCACAGAAGG | |||
| TNF-α | CATGAGCACTGAGAGCATGA | 170 | NM_214022.1 |
| CGATAACTTCGAAGTGCAGT |
GPx4, glutathione peroxidase 4; GPx1, glutathione peroxidase 1; CAT, catalase; SOD3, superoxide dismutase 3; SOD2, superoxide dismutase 2; SOD1, superoxide dismutase 1; NQO1, NAD(P)H quinone dehydrogenase 1; HO1, heme oxygenase 1; CYP1A1, cytochrome P450 family 1 subfamily A member 1; TXNRD1, thioredoxin reductase 1; GCLM, glutamate-cysteine ligase modifier; MGST1, microsomal glutathione S-transferase 1; UGT1A1, UDP glucuronosyl- transferase family 1 member A1; IL-1, interleukin 1; IL-6, interleukin 6; IL-8, interleukin 8; TNF-α, tumor necrosis factor α