Figure 2.

Characteristics of the LeFPS1 cDNA sequence. A, Nucleotide and deduced amino acid sequence of the LeFPS1 cDNA. Gray boxes correspond to the highly conserved regions of FPS (see text). Stop codons are indicated with asterisks and the putative polyadenylation site is written in italics. Underlined nucleotides denote a small ORF of unknown function. B, Autoradiography of in vitro transcription-translation products of LePFS1. LeFPS1 cloned in pBS was in vitro transcribed and translated in the presence of [³⁵S]Met as described in “Materials and Methods.” Radiolabeled products were separated on a 12% (w/v) SDS-PAGE and autoradiographed. “No RNA” and “LeFPS1” represent the transcription-translation respectively products in the absence and presence of LeFPS1. Numbers on the left indicate the molecular mass standards expressed in kD (New England Biolabs, Beverly, MA).