Figure 6.

Analysis of FPS mRNA accumulation during the development of tomato plant organs. A, Total RNA (15 μg per sample) prepared from fruits, leaves, flowers, and light or dark young seedlings (as indicated) were subjected to northern analysis. The blot was successively hybridized with a 182-bp cDNA fragment corresponding to the 3′-UTR of LeFPS1 (LeFPS1) and with the full-length LeFPS1 cDNA probe (LeFPS). Blots were respectively exposed for 12 (LeFPS1) and 2 d (LeFPS). Ribosomal RNA stained with ethidium bromide was used as a loading control. B, Total RNA (15 μg) extracted from flowers, and tomato leaves collected along the stem from the apex to the base of the plant were subjected to northern analysis using the full-length LeFPS1 cDNA as a probe. Ribosomal RNA stained with ethidium bromide was used as a loading control. The blot was exposed for 5 d. C, Total RNA (15 μg) prepared from tomato fruits harvested at different stages of development and ripening as indicated were subjected to northern analysis using the two probes described in A and with an actin probe (Germain et al., 1997), which was used as a loading control. Exposure times are as described in A. MG, Mature green; Hypo, hypocotyl; Cotyl, cotyledon; L breaker, late breaker; L turning, late turning.