Effects of baicalin treatment and the Western blotting results on the non-small cell lung cancer (NSCLC) cells, A549 and H1299. The upper part of this figure demonstrates the immunoblots for SIRT1, p-AMPK, AMPK, p-mTOR, mTOR, cleaved caspase-3 (c-caspase-3) encoded by the CASP3 gene, active matrix metalloproteinase (MMP)-2, active MMP-9 and GAPDH in cultured human NSCLC cells, A549 and H1299. The central part (columns) indicates the ratios of SIRT1/GAPDH (white columns), p-AMPK/AMPK (grey columns) and c-caspase-3/GAPDH (black columns) in cultured A549 cells and H1299 cells treated with baicalin and/or small interfering RNA (siRNA) silencing of the SIRT1 and AMPK genes. The columns on the bottom of this figure indicate the ratios of p-mTOR/mTOR (white columns), active MMP-2/GAPDH (grey columns), active MMP-9/GAPDH (black columns) in cultured A549 cells and H1299 cells treated with baicalin and/or small interfering RNA (siRNA) silencing of the SIRT1 and AMPK genes. * Indicates differences that were statistically significant.