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M1dG levels in nuclear DNA in RKO cells after a 24-h treatment with rotenone (100 nM), mitoTEMPO (10 μM), or TEMPOL (10 μM) alone or with rotenone (100 nM) and either mitoTEMPO (10 μM) or TEMPOL (10 μM). Data represent the mean ± S.D. of triplicate determinations. In RKO cells, basal levels of M1dG in genomic DNA was determined to be 1.5 adducts per 108 dG.
