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. 2018 Feb 5;46(7):3753–3763. doi: 10.1093/nar/gky050

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — The linker region is essential for NTP-dependent inhibition of ribosomal peptidyl transferase activity by the NTP hydrolysis-incompetent VgaA_LCEQ₂ mutant. The NTPase-incompetent VgaA_LCEQ₂ mutant does not rescue the puromycin reactivity of S. aureus 70S IC(MF) inhibited by 1 μM lincomycin (dark blue filled circles), and compromises protection by the wild type protein (dark blue circles, red fill) (A). The VgaA_LCEQ₂ mutant inhibits IC(MF) puromycin reactivity in the absence of antibiotics in the presence of 1 mM ATP (B and C) or GTP (C), but not 1 mM ADP, ATPγS or ADPNP (C). The VgaA_LCΔL mutant in which inter-ABC linker region (amino acids K199-S226) is substituted for a GSG linker neither protects the puromycin reactivity from 1 μM lincomycin (filled green circles), and its EQ₂ variant, VgaA_LCEQ₂ΔL, does not abolish the protective effect of the wild type protein (black circles with red fill) (D).