Figure 5.

Dram-seq using human Dicer, PACT, TRBP, and pre-miR-132. (A) In vitro dicing of 100 nM 5′ ³²P-radiolabeled pre-miR-132, pre-miR-27b, and their swap chimera pre-miRNAs by 6.5 nM HsDicer ± PACT or TRBP for 120 min. Representative gel image and length distributions revealed by quantification of gels (means of at least three independent trials) are shown. (B) Length distributions of miR-132a-3p isoforms produced from wild-type pre-miR-132 and its variants containing a single point mutation at nucleotide positions 40–58 by HsDicer ± PACT or TRBP, revealed by Dram-seq.