Table 1. Thermodynamic analysis of Leish4E-IP1 binding to LeishIF4E-1 as determined by isothermal titration calorimetry.

Titrants	Leish4E-IP11–100	Leish4E-IP11–52
K (cal/mol)	1.148 × 107 ± 6.645 × 106	1.03 × 108 ± 4.54 × 107
ΔH (cal/mole)	–3108 ± 80.36	–1.55 × 104 ± 281.8
ΔS (cal/mol/°)	–21.9	–15.3
ΔG (kcal/mole)	–2.56	–15.2
K D (nM)	87	9.7

Enthalpy changes (ΔH), entropy changes (ΔS), free energy changes (ΔG) and dissociation constants (K_D) derived from ITC measurements at 25°C.